Cloned (Comment) | Organism |
---|---|
functional expression of CYP106A2 in Escherichia coli strain BL21 from plasmid pET-CYP13 on the outer membrane with exposure on the surface without the external addition of the heme group but absolutely requiring the coexpression of TolC channel protein JW5503, because Escherichia coli uses a TolC-dependent mechanism to export heme into the growth media, where it can be scavenged by a surface-displayed apoenzyme | Priestia megaterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a adrenodoxin, heme, and cytochrome P450 containing enzyme | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + reduced adrenodoxin + O2 | - |
Priestia megaterium | 15beta-hydroxy-11-deoxycorticosterone + oxidized adrenodoxin + H2O | - |
? | |
abietic acid + reduced adrenodoxin + O2 | - |
Priestia megaterium | 12-hydroxyabietic acid + oxidized adrenodoxin + H2O | - |
? | |
imipramine + reduced adrenodoxin + O2 | - |
Priestia megaterium | desipramine + oxidized adrenodoxin + H2O | - |
? | |
additional information | enzyme reaction is coupled to NADPH oxidation, overview | Priestia megaterium | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP106A2 | - |
Priestia megaterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Priestia megaterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adrenodoxin | dependent on | Priestia megaterium | |
cytochrome P450 | - |
Priestia megaterium | |
heme | - |
Priestia megaterium |